The only '32' bit OSs that run 16 bit are in the 9x catagory (95,98,me). They are backward compatable for the older Win3x OS's.
If you went through the system files in the 9x series, you would see that they have a couple sets of system files, and some are for 16 bit, some for 32. You can't do this in anything later than ME, unless you buy some kind of a program that I'm not aware of.
Sig removed due to foul language. Please read the rules regarding the allowable content of sigs before reposting your COMPLIANT sig.