A few things to check:
Ensure the port you're opening is UDP and TCP
Ensure the port is forwarding to the computer that needs the port open (I'm assuming this is for a Minecraft server)
Ensure your server is running on that port (I know Bukkit allows you to change the port)
Ensure you can connect to the server locally (By using the servers internal IP address)
Make sure there are no other computers using that same port
If that doesn't work, your ISP may be blocking that port (unlikely, though. it isn't a commonly used port)
Try using a different port (My server runs on 25567 because I had it that way once and for some reason it just stuck
)
If you still have problems, post it here and I'll be happy to help you further.
EDIT: Also, make sure anyone connecting from outside your network connects using your external IP address. That can be obtained through a google search, "
What is my IP Address"